Program

Poster Presentations

Day 3, May 17（Thu.）　　Poster

3P-39　　PDF

Metabolic profiling of Hepatocellular Carcinoma by High-Definition Mass Spectrometry

(¹Tohoku Univ., ²Tohoku Univ., ³AMED-LEAP, ⁴Tohoku Univ., ⁵Univ. Tokyo, ⁶Univ. Tokyo)
^oDaisuke Saigusa^1,2,3, Koushi Nagai⁴, Uranbileg Baasanjav^3,5,6, Makoto Kurano^3,5,6, Kiyoshi Hasegawa⁶, Norihiro Kokudo⁶, Hitoshi Ikeda^3,5,6, Yutaka Yatomi^3,5,6, Yoshihisa Tomioka⁴, Junken Aoki^3,4

Hepatocellular carcinoma (HCC) accounts for approximately 90% of primary liver cancers and is the fifth most common cancer in the world. It is assumed that the number of HCC cases will continue to increase, and biomarkers that are related to the degree of tumor progression and cellular characteristics and that can provide clinical information linked to treatment are required. Metabolomics has been developed as one of the post-genomic research fields and is a promising avenue for discovery of HCC biomarkers. Especially, the global metabolomics (G-Met) can analyze metabolic pathways and obtain new knowledge, such as the detection of unknown metabolites. However, G-Met has following problems: (1) the comprehensiveness and reproducibility of the measurement method to cover a wide range of metabolites; (2) the identification of compounds; and (3) the acquisition of information relating to the spatial localization of the compounds. Regarding problem; (1) we used a mixed-mode column that is based on a combination of ion exchange and reversed phase technologies; (2), we used high-definition mass spectrometry, which can separate ions by ion mobility; (3) we used desorption electrospray ionization-MS imaging, which incorporates an electrospray probe and LC-MS.