Oral Sessions
(Day1, Day2, Day3, Day4)
Poster Presentations
(Day1, Day2, Day3, Day4)
Luncheon Seminars
(Day1, Day2, Day3, Day4)
Poster Presentations
- Day 1, May 15(Tue.) Poster
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1P-12 PDF
Development of a sensitive and accurate analytical method for sugar phosphates and its application to 13C-metabolic flux analysis of cancer cells
A method for sugar phosphates analysis was developed using GC-NCI-MS. Baseline separation of sugar phosphate isomers and detection of molecular ion in ten nano-molar range were achieved. The method was applied to the measurement of 13C-labeling of sugar phosphates for 13C-metabolic flux analysis of MCF-7 human breast cancer cell line. Employment of 13C-labeling of sugar phosphates improves the flux estimation of bidirectional reactions in glycolysis and the pentose phosphate pathway.