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Poster Presentations
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Luncheon Seminars
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- Day 1, May 15（Tue.） 14:25-14:45 Room B (Seiun 1)
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1B-O1-M-1425 PDF
Genotyping of Poisonous Organisms by Polymerase Chain Reaction Followed by Liquid Chromatography/Quadrupole-Orbitrap Mass Spectrometry
Serious food poisoning caused by poisonous organisms has occurred due to misidentification and inappropriate preparation of them. Moreover, intentional assaults and homicide using the organisms such as pufferfish and poisonous plants did occur. In such cases, police laboratories like us are responsible for the identification of the species of the causative organism, as well as the toxic components. For rapid and accurate genotyping for forensic purpose, we developed a method for determining the monoisotopic mass of a PCR product by LC/ESI-MS using a commercially-available reverse-phase silica monolith column and a Q Exactive high resolution mass spectrometer. The approach developed has been applied for the differentiation of toxic pufferfish species based on the use of the PCR restriction fragment length polymorphism (RFLP) method, which is the first example for species differentiation of animals using MS. A major poisonous plant (Cicuta virosa) and the similar edible one (Oenanthe javanica) were also genotyped successfully.