日本質量分析学会 第66回質量分析総合討論会

Program

Poster Presentations

Day 4, May 18(Fri.)  Poster

timsTOF Pro with PASEF for shotgun proteomics

(Bruker Japan)
oRyo Kajita, Yoshihiko Takinami, Takashi Nirasawa

Proteomics researchers require mass spectrometers with increased speed and sensitivity to address the complex mixture of peptides eluting from nano-flow LC columns in data dependent shotgun proteomics workflows. The “Parallel Accumulation Serial Fragmentation” method (PASEF) for trapped ion mobility spectrometry (TIMS) quadrupole time of flight (QTOF) instruments, enabled five to ten times faster data dependent acquisition of fragment ion spectra. The benefits of the PASEF method for identifying more proteins in less time and with less sample in data-dependent shotgun proteomics experiments will be demonstrated.