Program

Oral Sessions

Day 4, May 18（Fri.）　15:05-15:25　Room B (Seiun 1)

4B-O2-P-1505　　PDF

Surface Proteome Profiles Meet Adipocyte Commitment

(Kyoto Univ.)
^oHsin-yi Chang, Naoyuki Sugiyama, Yasushi Ishihama

Adipose tissue is a major endocrine organ to secret signaling proteins that regulate whole-body energy homeostasis. To date, three forms of fat, white adipose tissue storing energy in form of lipids, brown adipose tissue dissipating energy as heat, and beige adipose tissue, another energy-dissipating adipose tissue arisen from white ones have been discovered. Although factors determining adipose functions have been revealed at a broad level from receptor activation to gene expression, the upstream regulations initiated from extracellular stimuli remain exclusive. To explore how cell fate commitment is achieved by microenvironment networking from the viewpoints of surface protein composition and dynamics, we employed chemical proteomic approaches for surfaceome targeting through hydrazide chemistry1) and primary amine labeling and identified by LC/MS/MS from three terminal differentiated adipocytes (Fig 1). 496 proteins from 1027 glycopeptides containing at least one N-X-S/T domain and 1569 peptides with chemically modified lysine were identified. We identified more than 50% previously annotated surface proteins2) and revealed 58 Clusters of Differentiation antigens and 8 class I histocompatibility antigens. Here we report a panel of proteins which exclusively expressed in brown, white or beige adipocytes, defining a primed role of surfaceome exerting unique physiological function in differentiated adipocytes.