Program

Oral Sessions

Day 4, May 18（Fri.）　10:15-10:30　Room B (Seiun 1)

4B-O1-P-1015　　PDF

Drought stress characterization of tolerant and sensitive barley genotypes through Proteomics analysis

(¹UAJK, ²PMASAAUR, ³NARC, ⁴UoT)
^oRehana Kausar¹, Muhammad Arshad², Armghan Shahzad³, Setsuko Komatsu⁴

Plant productivity is severely impaired by drought stress and it is an important factor limiting barley yield as well. Proteomics technique was used to investigate the initial response of barley under drought stress. Three-day-old barley seedlings of tolerant genotype 004223 and sensitive genotype 004186, were treated with 20% polyethylene glycol and by withholding water to impose drought. Proteins were extracted after three days of treatments, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. In response to drought among the common proteins between tolerant and sensitive genotype, Photosystem I reaction centre II was decreased and Vacuolar proton ATPase subunit E was increased. In the tolerant group, many of the Metabolism related proteins were increased under drought stress, however such type of proteins were decreased in sensitive genotypes. These results indicate that chloroplastic metabolism and energy related proteins might play a significant role in the adaptation process of barley seedlings under drought stress.