Oral Sessions
（Day1, Day2, Day3, Day4）
Poster Presentations
（Day1, Day2, Day3, Day4）
Luncheon Seminars
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Poster Presentations
- Day 3, May 17（Thu.） Poster
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3P-36 PDF
Development of an analytical method for mitochondrial metabolites using photoaffinity labeling
Mitochondria are essential organelle for energy production and cellular existence and they regulate many cellular functions. In order to clarify the detailed mechanisms of cell phenomena, accurate analytical methods for mitochondrial metabolites have been demanded. However, the conventional metabolic approaches are difficult to know the localization of cellular metabolites because these methods analyze large amounts of cells or tissues as homogenized samples. In this study, in order to analyze metabolites located in the mitochondria, we developed an analytical method for metabolites in mitochondria by using mitochondrial localized photoaffinity probe. Our synthesized probe has both diazirin moiety as photoaffinity unit and rhodamine B as a mitochondrial affinity unit, respectively. From the result of fluorescence microscope observation, it was confirmed that this probe was localized in mitochondria of HepG2 cells. Thus, the method would be useful for selective analysis of mitochondrial metabolites.