Program

Poster Presentations

Day 3, May 17（Thu.）　　Poster

3P-01　　PDF

Detection of intact protein molecules by nanoLC-MS

(¹Nagoya Univ., ²Shinwakako)
^oKeiko Kano¹, Hiroshi Kobayashi², Hiroo Wada², Keiko Kuwata¹

Protein identification is usually done by “bottom-up” proteomics, in which the proteins are digested by trypsin prior to LC-MS/MS analysis. However, in case of truncated protein for example, clarification of the terminal amino acid position is often difficult by “bottom-up” proteomics. Although using conventional LC-MS allows us to detect truncated proteins efficiently, it is difficult when the amount of truncated proteins is too low. Therefore, it is important to detect intact protein molecules by nanoLC-MS. In this presentation, a monolithic silica capillary column that was developed by Shinwa Chemical Industries Ltd. was connected to nanoLC-MS to evaluate the performance using several proteins.