Program

Poster Presentations

Day 2, May 16（Wed.）　　Poster

2P-34　　PDF

Liver Tissue Heat Stabilization is Efficient for Differential Metabolome Analysis of Tiny Dissected Normal and Metastatic Cancer Tissues.

(¹Kyoto Univ., ²Keio Univ., ³Doshisha Univ.)
^oKohta Iguchi¹, Koshiro Morino¹, Hiroto Nishino¹, Yuki Sugiura², Masaya Ikegawa³, Shinji Uemoto¹

Heat Stabilization (HS) allows us to provide nearly in vivo information about metabolites, which are easily susceptible to ex vivo degradation. We previously reported that the appropriate visualization of adenosine triphosphate (ATP) in the liver using imaging mass spectrometry (IMS) could be obtained from HS fresh samples not from HS frozen ones. In this study, comprehensive quantitative approach to the mouse liver revealed that the various metabolites including ATP could be classified according to the presence or absence of HS treatments. In the metastatic liver cancer rat models, the cancer-specific pathway was elucidated by the metabolic flux analyses using ¹³C-labeled glucose/glutamine tracers. We could also visualize the heterogeneity of the metabolic processes on the cancer and non-cancer tissues using IMS.