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Luncheon Seminars
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Poster Presentations
- Day 2, May 16（Wed.） Poster
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2P-16 PDF
Physicochemical, Proteome and Metabolome studies to Determine Water Use Efficiency in Grape
Water stress affects productivity, disease tolerance, and enological characteristics of grape. Regulated water deficit conditions resulted in changes in protein composition affecting specific metabolite pathways. Studies conducted on physiological, proteome and metabolome changes to regulated water stress to determine the pathways associated with the stress tolerance mechanism. Grape cultivars Blue lake, and Blanc du Bois were maintained under green house conditions and exposed to regulated water stress. Berry samples were collected from both irrigated and stressed plants at weekly intervals. Physicochemical parameters and metabolites such as total titratable acidity, total soluble solids, total proteins were separated by 2-DE followed by MALDI TOF/TOF mass spectrometry. Various metabolites were quantitatively analyzed using triple-quadrupole MS-based data acquisition mode. A linear decrease of the photosynthesis rate, and IAA, while abscisic acid, superoxide dismutase increased with the decrease in water potential in both cultivars was observed. MS/MS studies identified 79 differently expressive proteins, involved in, signal transduction, response to stimulus, calcium signaling pathway, anti oxidative systems and related to epigenetic modifications. Kinase like protein was increased in Blue Lake suggesting a chromatin modification. Tolerant characteristics of cultivar Blue Lake is associated with the more abundance in metabolites involved in epigenetic modifications.