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2C-O1-P-1005 PDF
Assessment by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the effects of preanalytical variables on serum peptidome profiles following long-term sample storage
Sample collection and handling procedures can have a profound impact on the serum proteome, and we previously demonstrated that the time between venipuncture and serum preparation affected serum peptidome patterns1). Several previous studies used MALDI-TOF MS to assess the pre-analytical stability of serum proteomes subjected to different storage conditions for a maximum period of 3 months. There is little clear information regarding how low molecular weight peptides in serum are affected by long-term storage. Therefore, in the present study, we tested the effects of long-term storage for up to 12 months on serum proteome and peptidome profiles2). Aliquots of serum samples were pretreated with weak cation exchanger magnetic beads using an automated ClinProtRobot system (Bruker Daltonics, Lepzig, Germany) and then analyzed by MALDI-TOF MS. In the present study, the peak patterns remain unchanged for samples stored at −80°C or in liquid nitrogen for 12month, whereas the intensities of as many as 20 MS peaks changed markedly during storage at −20°C for 3month. There was no difference in the relative intensity of each peak between samples thawed on ice, at room temperature, or at 37°C when stored for 3, 6, or 12 months at −80°C or in liquid nitrogen. Based on our results, we would recommend to use samples stored at -80 °C or in liquid nitrogen instead of those stored at -20 °C for biomarker discovery study targeting serum peptides.