Program

Oral Sessions

Day 2, May 16（Wed.）　16:00-16:20　Room B (Seiun 1)

2B-O2-M-1600　　PDF

New Insights in 20keV High-Energy CID of Peptides using MALDI-TOF/TOF MS

(¹Yokohama City Univ., ²Shimadzu)
^oMitsuo Takayama¹, Sadanori Sekiya², Shinichi Iwamoto², Koichi Tanaka²

Positive-ion CID spectra of peptides obtained with 20keV high-energy TOF/TOF MS have been analyzed from the standpoints of selective/enhanced cleavage of Asp/Glu/Cys-Xxx, Xxx-Pro and Gly-Xxx residues by comparing with low-energy CID. The high-energy CID spectrum of a model peptide BSA1-48 containing Asp, Glu, Cys, Pro and Gly residues showed preferential b- and y-ions originated from the cleavage at C-N bond of Asp-Xxx residues, which is a characteristic of low-energy CID. The selective/enhanced cleavage of Asp-Xxx residues in high- and low-energy CID could be explained by assuming the pre-formation of an intramolecular hydrogen-bonding state (Fig. 1left).