Program

Oral Sessions

Day 2, May 16（Wed.）　11:05-11:25　Room B (Seiun 1)

2B-O1-P-1105　　PDF

Soybean Omics to Reveal the Flooding-Tolerant Mechanism at Initial Stage

(¹Univ. Tsukuba, ²Maebashi Inst. Tech.)
^oSetsuko Komatsu¹, Katsumi Sakata²

Soybean is sensitive to flooding stress, which markedly reduces its growth. To identify the mechanism of flooding tolerance at initial stage in early-stage soybean, omics techniques were used. Flooding tolerant mutant line and abscisic acid (ABA)-treated soybean, which exhibited flooding tolerant phenotype, were used as materials. Two-day-old soybeans were flooded for 3 h as initial flooding stress and roots were collected for proteomic as well as metabolomic and transcriptomic analyses. Data were analyzed using functional categorization, cluster separation, KEGG software, and in silico protein-protein interaction analyses. Furthermore, commonly changed metabolites, proteins, and genes between mutant and ABA-treated soybeans were considered as flooding-tolerance related candidate factors. Finally, omics results were integrated to analyze the flooding tolerant mechanism in soybean at initial stage. Proteomic results indicate that flooding tolerance at initial stage in early-stage soybean is through protecting newly synthesized proteins and inhibiting cell wall loosening. Transcriptomic results indicate that cytochrome P450 77A1 is suppressed by uniconazole treatment and that this inhibition may enhance soybean tolerance to flooding stress. Additionally, integrated omics suggests that fructose might be the critical metabolite through regulation of hexokinase/phosphofructokinase and the regulation of energy metabolism might be crucial step to confer initial-flooding tolerance in early-stage soybean.