Program

Oral Sessions

Day 2, May 16（Wed.）　10:45-11:05　Room B (Seiun 1)

2B-O1-P-1045　　PDF

Insights into Nanoparticles Interaction with Soybean at the Proteomics Level

(¹QAU, Pakistan, ²Tsukuba Uni. Japan)
^oGhazala Mustafa^1,2, Setsuko Komatsu²

The recent trends of nanoparticles (NPs) usage in agriculture sector is captivating. To investigate the possible interactions between NPs and crops under flooding stress, the label-free proteomic technique was used. Al2O3 and Ag-NPs under flooding stress enhanced soybean growth compared to ZnO-NPs. Among the various sizes and concentrations, 15 nm Ag-NPs promoted soybean growth. The Ag-NPs treated soybeans have experienced less oxygen-deprivation stress through the production of less cytotoxic by-products of glycolysis. The different sizes of Ag-NPs affected the soybean growth under flooding by regulating the proteins related to amino acid synthesis and wax formation. The 30-60 nm Al2O3-NPs improved the soybean growth compared to other sizes and concentrations. Al2O3-NPs-responsive proteins were related to protein synthesis/degradation, glycolysis, and lipid metabolism. Al2O3-NPs promoted the growth of soybean under flooding stress by regulating energy metabolism and cell death. Mitochondrion was the target organelle of Al2O3-NPs. Mitochondrial proteomic analysis revealed that the abundance of voltage-dependent anion channel protein was increased in soybeans with 135 nm Al2O3-NPs. Therefore, Al2O3-NPs of various sizes affect mitochondrial proteins under flooding stress by regulating membrane permeability and tricarboxylic acid cycle activity. Furthermore, the energy regulation and membrane permeability might be the principal component for NPs interaction with soybean.