Program

Poster Presentations

Day 1, May 15（Tue.）　　Poster

1P-12　　PDF

Development of a sensitive and accurate analytical method for sugar phosphates and its application to ¹³C-metabolic flux analysis of cancer cells

(¹Osaka Univ., ²Shimadzu, ³Osaka Univ.)
^oNobuyuki Okahashi¹, Kousuke Maeda¹, Shuichi Kawana², Junko Iida^2,3, Hiroshi Shimizu¹, Fumio Matsuda¹

A method for sugar phosphates analysis was developed using GC-NCI-MS. Baseline separation of sugar phosphate isomers and detection of molecular ion in ten nano-molar range were achieved. The method was applied to the measurement of ¹³C-labeling of sugar phosphates for ¹³C-metabolic flux analysis of MCF-7 human breast cancer cell line. Employment of ¹³C-labeling of sugar phosphates improves the flux estimation of bidirectional reactions in glycolysis and the pentose phosphate pathway.