Program

Oral Sessions

Day 1, May 15（Tue.）　14:45-15:05　Room A (OrBit Hall)

1A-O1-M-1445　　PDF

iMPAQT ver.2.0: a scalable and flexible platform for the quantification of proteins of interests

(Kyushu Univ.)
^oMasaki Matsumoto, Keiichi Nakayama

Mass-spectrometry based proteomics is a powerful tool for discovery of biologically important proteins through protein expression profiling, determination of protein-protein interactions, and identification of post-translational modifications, such as phosphorylation. Beside the adoption of such discovery proteomics, the advent of technologies for targeted proteomics allows us to measure the dynamics of any proteins of interest under a variety of conditions as well as to estimation of the absolute abundance or stoichiometry of proteins in a given network. Multiplexed targeted proteomics assays of high reproducibility and accuracy can provide insight at the quantitative level into entire networks that govern biological phenomena or diseases.
We have developed a new targeted proteomics platform-in vitro proteome-assisted MRM for protein absolute quantification (iMPAQT)-using >18,000 human recombinant proteins for genome-wide protein absolute quantification (Matsumoto et al., Nature Methods, 2017). We have now been developing a second version of iMPAQT to improve the scalability and flexibility for measurement of multiple proteins across a large number of samples.