Sweets Seminar
Day 2, June 11（Thu.） 14:30-15:00 Room C (4F 413)
- 2C-SW-1430
Improving LC/MS Analysis through Optimized Column Design: Adsorption Suppression and Novel Stationary Phases
(ChromaNik Technologies)
oRyuji Koyama, Tomoyasu Tsukamoto, Norikazu Nagae
In LC/MS analysis, column performance strongly influences separation efficiency, peak shape, sensitivity, and reproducibility. Undesired adsorption arising from residual silanol groups, metal surfaces, or stationary phase chemistry can lead to peak tailing, signal loss, and poor analytical reliability, particularly for basic compounds, polar molecules, and biomolecules. Therefore, column design aimed at suppressing such interactions is essential for achieving robust LC/MS analysis.
This presentation introduces several column designs aimed at reducing adsorption in LC/MS applications. Examples include peptide-oriented phases (C18, C9), aromatic stationary phases such as pentafluorophenyl (PFP) and biphenyl phases, and columns based on ethylene-bridged hybrid silica. These examples highlight the importance of column design in reliable LC/MS analysis.