Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-41
Direct Native Mass Spectrometry of Endogeneous Large Protein Assemblies in Crude Cell Lysates Using High-Resolution Multi-Turn TOF-MS
(1Yokohama City Univ., 2Shimadzu)
oMichiko Tajiri1, Yusuke Tateishi2, Qiuyi Wang2, Sayaka Hokazono1, Hiroko Morinaga2, Hiroyuki Miura2, Masaru Nishiguchi2, D Okumura2, Tsuyoshi Konuma1, Satoko Akashi1
Native mass spectrometry (nMS) is a powerful technique for observing non-covalently bound protein complexes while preserving their higher-order structures. However, because target complexes are typically high-mass species, achieving both high mass resolution and sufficient sensitivity at elevated m/z values remains a significant technical challenge. In this study, we directly analyzed endogenous protein assemblies from crude cell lysates without purification as a step toward understanding proteins in their native environments. Since cell lysates contain various salts and other impurities that cause peak broadening and signal suppression, an instrument with sufficient resolving power and sensitivity is required. To address this challenge, we employed a high-resolution multi-turn (MT) TOF-MS system optimized for nMS and successfully detected endogenous protein assemblies up to approximately 150 kDa directly from crude cell lysates. Comparative analyses with a conventional Q-TOF instrument were also performed, and differences in spectral features will be presented.