Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-30

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Analysis of the Extraction and Ionization Processes in Tapping-Mode Scanning Probe Electrospray Ionization (t-SPESI) by Spray Current Measurement

(¹UOsaka, ²Univ. Yamanashi)
^oMengze Sun¹, Yoichi Otsuka¹, Lee Chuin Chen², Michisato Toyoda¹

Tapping-mode scanning probe electrospray ionization (t-SPESI) is an ambient sampling and ionization method for mass spectrometry imaging (MSI) that uses a small amount of solvent delivered by an oscillating capillary probe. When the probe tip contacts the sample surface, a solvent liquid bridge is formed between them for extraction. After probe retraction, the extracted analytes undergo electrospray ionization. When conducting t-SPESI-MSI, an appropriate voltage adjustment is necessary to achieve stable sampling and ionization. Because the adjustment has been performed manually, clear criteria for optimization are required. In this study, we present a current measurement method for t-SPESI and propose a strategy for adjusting the voltage to regulate the liquid bridge size and achieve stable ESI.

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