Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-24

PDF

Analysis of Intercellular Glycometabolic Activity Using Live Single-Cell Mass Spectrometry

(¹Meijo Univ., ²Univ. Shizuoka, ³Ajinomoto)
^oRyosuke Fukui¹, Seiya Yonedu¹, Aogu Furusho², Akiho Murai³, Kotoe Nakasha³, Akihiro Arakawa³, Sachise Karakawa³, Eiji Sugiyama¹, Kenichiro Todoroki², Hajime Mizuno¹

Detailed analysis of cells is essential for understanding biological phenomena and disease mechanisms. In particular, cancer cells exist in heterogeneous states. Therefore, accurate understanding of cancer cell phenotypes requires analysis of metabolic states at the single-cell level. In this study, we treated cancer cells with the fluorescent glucose analog 2-NBDG to visualize intercellular glucose metabolic activity. We sampled cells with high and low glucose metabolic activity, as indicated by differing fluorescence intensities, and analyzed them by mass spectrometry. The results of TAHS derivatization and LC-MS analysis of single-cell samples showed that some amino acid peaks were detected. These amino acid peak intensities increased when the number of sampled cells was increased from one to five. This confirmed that intracellular amino acids were detected within a single-cell. In addition, several amino acids levels correlated with cellular fluorescence intensities. These results suggest that intracellular metabolite levels can be compared at the single-cell level based on glucose metabolic activity in hematological cancer cells.

---