Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-23

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Optimization of MALDI-MSI-based single-cell lipidomics for human peripheral blood immune cells

(¹RIKEN IMS Lab. for Metabolomics, ²RIKEN IMS Lab. for Autoimmune Diseases, ³AB SCIEX, ⁴Keio Univ. Pharm.)
^oYumiko Ono¹, Haruki Uchino^1,4, Yosuke Isobe^1,4, Akari Suzuki², Ushio Takeda³, Manami Takeuchi³, Keiko Myouzen², Gyo Inoue², Kazuhiko Yamamoto², Makoto Arita^1,4

Lipid metabolism plays central roles in immune cell function, yet lipid heterogeneity at the single-cell level remains poorly understood. Here we applied matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to perform single-cell lipidomics of human immune cell subsets. Twelve immune cell populations were isolated from peripheral blood of six healthy donors and analyzed using dual-polarity MALDI-MS imaging together with bulk LC–MS/MS lipidomics. Single-cell analysis detected more than 180 lipid species across approximately 40,000 cells and revealed pronounced lipid metabolic heterogeneity within immune cell subsets.

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