Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-18

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Establishment of a MALDI-MSI platform for three-dimensional analysis of spheroids

(¹Teikyo Univ., ²Teikyo Univ., ³Teikyo Univ., ⁴Tsurumi univ., ⁵Tohoku Univ.)
^oMizuha Hishinuma¹, Hirokazu Ohata², Arisa Ihsii³, Tomoko Fukuuchi¹, Noriko Yamaoka¹, Tadafumi Kawamoto⁴, Kouji Okamoto², Daisuke Saigusa^1,5

Three-dimensional (3D) cell culture more faithfully reproduces the in vivo microenvironment than conventional two-dimensional systems, reflecting cell–cell interactions and spatial metabolite gradients. Understanding these spatial dynamics is essential for elucidating cancer progression and drug resistance. However, achieving spatially resolved molecular analysis at the cellular level remains technically challenging. In this study, we developed a MALDI-MSI approach to visualize metabolites in HCT116-derived spheroids. Serial sections (8 µm) were prepared using the Kawamoto method and analyzed with iMScope QT. Phosphatidylcholine was localized within spheroids. Although 1 µm imaging initially yielded insufficient signals, optimization of laser parameters enabled 2 × 1 µm resolution, exceeding the nominal 5 µm specification. These findings demonstrate high-resolution metabolic imaging in 3D spheroid models.

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