Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-16

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Lipid Profiling of Extracellular Vesicles Derived from Muse Cells

(¹Tohoku Univ., ²Tohoku Univ., ³Tohoku Univ., ⁴Teikyo Univ., ⁵Tohoku Univ.)
^oYuko Iwasaki¹, Daisuke Ando¹, Eiji Hishinuma^2,3, Naomi Matsukawa^2,3, Daisuke Saigusa^2,4, Rashad Sherif⁵, Kuniyasu Niizuma^1,3,5

Multilineage-differentiating stress-enduring cells (Muse) cells are endogenous stem cells with tissue-repair capabilities. Their therapeutic effects have been suggested to be mediated, at least in part, by extracellular vesicles (EVs), however, the lipid composition of Muse cell-derived EVs remains unclear. In this study, we comprehensively analyzed the lipid composition of EVs derived from Muse cells and compared it with that of EVs from non-Muse cells. Muse cells were isolated as SSEA-3-positive cells, and the SSEA-3-negative fraction was used as non-Muse cells. EVs were collected from culture supernatants of both cell populations. Lipidomics was performed using UHPLC-FTMS and UHPLC-MS/MS. The results revealed group-dependent differences in EVs staining and identified multiple lipid species that differed significantly between Muse-derived and non-Muse-derived EVs (P < 0.05). These finding provide, to our knowledge, the first comprehensive characterization of the lipid profile of EVs derived from Muse cells and may contribute to understanding EV-mediated mechanisms underlying Muse cell-based tissue repair.

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