Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-13
Proton Transfer Reaction of Substance P
(Yokohama City Univ.)
oTomoya Sugiyama, Koki Sasano, Reo Kodaira, Shinji Nonose
Proton transfer reactions of the neuropeptide Substance P in the gas phase were investigated using a laboratory-built tandem mass spectrometer. Doubly protonated Substance P ions were generated by electrospray ionization and reacted with 1,5-pentanediamine in a gas cell. The dependence of the reaction on DC voltage applied to the octapole ion guide (OPIG-DC), temperature, buffer gas conditions, reaction time, and deuteration was examined. The reaction was most efficient at low OPIG-DC voltages and was suppressed at higher voltages. Increasing temperature and deuteration both led to reduced reaction rates. These results indicate that the proton transfer reactivity of Substance P is strongly governed by the internal energy of the ion.