Poster Presentations
Day 1, June 10（Wed.） Room P (5F 501+502)
- 1P-07（3C-P2-1458）
Metabolomic Profiling of Extracellular Vesicles in Cancer and Cellular Senescence
(Keio Univ.)
oRyosuke Hayasaka, Akiyoshi Hirayama
Extracellular vesicles (EVs) are lipid bilayer-containing vesicles less than 1 μm. In cancer research, EVs derived from cancer cells have been reported to contribute to cancer progression such as angiogenesis and immune suppression. Analysis of EV contents has been limited to nucleic acids such as microRNA, mRNA, and DNA, as well as proteins and lipids, leaving many aspects of hydrophilic metabolites unclear. In this presentation, we report the establishment of a metabolomics analysis system suitable for EVs and demonstrate that the metabolome within EVs fluctuates in response to cancer and cell senescence. We performed Hydrophilic metabolomic analysis using capillary IC-MS and LC-MS, and lipidomic analysis using SFC-QqQMS on EVs. We identified 85 hydrophilic metabolites and 575 lipids from EVs derived from HCT116 cells. 2-hydroxyglutarate, an oncometabolite involved in carcinogenesis and cancer progression, was found within EVs. In cellular senescence, a hallmark of aging, EVs are released as part of the senescence-associated secretory phenotype and contribute to chronic inflammation and age-related diseases. Using our analytical system, we found alterations in EV metabolomic profiles following senescence induction. We are advancing technology to enhance metabolite identification in EVs and will present the current progress in this talk.