Program

Poster Presentations

Day 1, June 10（Wed.）　　Room P (5F 501+502)

1P-01（1B-S2-1640）

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Development of Intracellular Pharmacokinetic Evaluation Method Using a Single-cell Nano-delivery System

(¹Meijo Univ., ²Univ. Shizuoka, ³Yokogawa Electric)
^oMitsuki Watanabe¹, Ryudai Yamamoto¹, Akane Ikoma¹, Takuma Yanagisawa^1,2, Kazumasa Kimura³, Yuta Terui³, Masafumi Iharada³, Fumitaka Kamachi³, Hironori Takai³, Eiji Sugiyama¹, Hajime Mizuno¹

In this study, we developed a method for the direct intracellular injection of drugs using a single-cell nano-delivery system. We also established a single-cell mass spectrometry approach to analyze the injected drugs and their metabolites within individual cells. A mixture of amiodarone and propidium iodide (PI) was injected into HepG2 cells under microscopic observation using the single-cell nano-delivery system. The injected cells survived for more than 24 hours, indicating that the injection procedure exerted minimal effects on cell viability. After 24 hours of incubation, cells exhibiting PI-derived fluorescence were collected using a single-cell sampling system. As a result of mass spectrometry analysis of these cell samples, injected amiodarone and its metabolite, N-desethylamiodarone peaks were detected. These results show that the developed methods enable the direct intracellular delivery of drugs and subsequent metabolic analysis at the single-cell level.

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