Symposium Sessions
Day 1, June 10（Wed.） 14:08-14:26 Room C (4F 413)
- 1C-S1-1408
Dissecting Plant Peptide Signaling via MS-Based Multi-Omics at Single-Cell and Spatial Resolution
(NCHU, Taiwan)
oYing-Lan Chen
Peptides are essential signaling molecules mediating intercellular communication in plants. Yet their low abundance, rapid turnover, mobility and strong tissue specificity make peptides difficult to study using conventional approaches. By applying our developed mass spectrometry–based multi-omics platform integrating peptidomics, phosphoproteomics, and metabolomics, we systematically characterized stress-responsive peptide signaling in plants. Our analyses revealed that mechanical wounding induces the production of CAPE peptides, which function as long-distance mobile signals to activate plant defense responses. In addition, we identified a novel vascular sap peptide, ASAP, that is associated with phosphorylation-mediated signaling during tension wood formation under mechanical stress. Recent advances in high-resolution mass spectrometry further enable peptide discovery at single-cell and spatial resolution. Integrating single-cell multi-omics with spatial mass spectrometry imaging allows the reconstruction of peptide signaling networks and the localization of peptide activity within intact tissues. These approaches uncover evolutionarily conserved peptide regulators involved in stress adaptation and provide a molecular framework for improving crop resilience under environmental challenges.