Symposium Sessions
Day 1, June 10（Wed.） 15:00-15:20 Room B (4F 411+412)
- 1B-S1-1500
Development of a t-SPESI System for Single-Cell Mass Spectrometry Imaging of Biological Tissues
(1UOsaka, 2UOsaka)
oYoichi Otsuka1, Takao Yasuda1, Tasuku Kato1, Yang Zhou1, Shuichi Shimma2, Michisato Toyoda1
Single-cell mass spectrometry imaging (MSI) of biological tissues is limited by low ion yields at micrometer-scale spatial resolution and signal instability during prolonged acquisition. We developed a tapping-mode scanning probe electrospray ionization (t-SPESI) system to improve ion transfer efficiency and operational stability. Shortening the ion transfer tube significantly enhanced sensitivity by increasing ion transmission efficiency. Signal instability caused by adsorption on micrometer-scale quartz probes was mitigated by introducing a fluorinated molecular layer onto the probe surface. The optimized system enabled stable long-term MSI acquisition and revealed fine lipid distributions in mouse brain tissue, demonstrating the feasibility of practical single-cell MSI using t-SPESI.