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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-14
Ambient Ionization Mass Spectrometry for Rapid Characterization of Biomarkers on Inflamed Skin
(NSYSU, Taiwan)
oShu-Yao Lin, Jentaie Shiea
Histamine is a biogenic amine synthesized in the human body, playing a crucial role in immune response. When exposed to allergens or skin injuries, such as food allergens and insect bites, immune cells release higher concentrations of histamine to recruit additional immune cells, defending against foreign antigens. This robust release initiates physiological responses, including inflammation, itching, rashes, and hives. Traditional clinical allergen analysis relies on invasive biopsy methods that require extensive sample preparation, causing patient discomfort, infection risks, and lengthy analysis times (typically 3-6 hours).
In contrast, thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS) is a rapid (<1 minute) 1,2), non-invasive method that allows direct sampling without preparation. In this study, we utilized TD-ESI/MS to detect histamine secretion on the skin surface, investigating responses to two exogenous stimuli: (1) coating the subject's forearm with taro (Alocasia spp.) and (2) mosquito bites on the subject's leg. Results indicated that histamine signal intensity changed based on skin sensations and inflamed locations. This methodology overcomes traditional limitations by enabling real-time cutaneous biomarker analysis, providing foundational data for developing non-invasive diagnostic platforms in precision dermatology.