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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-07
Quantitative analysis of tridecylcyclohexane in mice using SPME-GC/MS
(1GSS Univ. Osaka, 2NIBIOHN, 3IPR Univ. Osaka)
oJunichi Osuga1, Fuminori Iijima2, Yoshiatsu Aomine3, Toyomasa Katagiri2, Michisato Toyoda1
Solid phase microextraction (SPME) is a well-known technique that can be used to concentrate organic compounds in the gas or aqueous phase and perform sensitive analysis. On the other hand, incomplete Freund's adjuvant (IFA), which is composed of mineral oil as main component containing many kinds of hydrocarbons, is well known for its ability to activate the immune system. IFA has also been used for more than 8 decades to induce a variety of autoimmune diseases in mice. Last year, Iijima N. et al. discovered that tridecylcyclohexane (TDCH) in mineral oil is one of the key compounds in causing autoimmune disease in mice following immunization with self-antigens in IFA. (Eur. J. Immunol. 2024; 54:2350957) Hydrocarbons in mineral oil could be analyzed by GC/MS. However, they are expected to be present in biological tissues with very small amounts, hence it is desirable to analyze them with as high sensitivity as possible. For this reason, we investigated multiple analytical conditions using SPME-GC/MS with standards diluted in isooctane. As a result, we found that the equilibration time was less than 10 minutes, the reproducibility was within 3%, and the linearity from 0.1 ppb to 10 ppb was R2 > 0.999.