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Oral Sessions
Day 4, June 25（Wed.） 14:10-14:25
Room C (Top of Yaima)
- 4C-O2-1410
Remodelling of the yeast lipidome as an adaptation to temperature
(1QUT, 2UOW)
oStephen Blanksby1, Rangika Perera1, Reuben Young2, Aurelie Benefield1, Sonia Henriques1, Berwyck Poad1
Cellular membranes are composed of proteins that are embedded within a lipid bilayer. The biophysical properties of cellular membranes play a crucial role in facilitating cellular functionality and ensuring cellular viability under diverse environmental circumstances. The membrane lipids are primarily responsible for determining biophysical properties, including membrane thickness, membrane viscosity, membrane bending rigidity, membrane compressibility and membrane fluidity. Glycerophospholipids are the key lipid constituents in cellular membranes which not only serve as key building blocks but also influence the biophysical properties of cellular membranes. Herein, a budding yeast, Saccharomyces cerevisiae, is used as an example to measure the responsiveness of membrane lipid composition to growth temperature via a shotgun lipidomics platform. Targeted lipids were mass-selected and subjected to ozone-induced dissociation (OzID), collision-induced dissociation (CID), and sequential collision-induced dissociation/ozone-induced dissociation (CID/OzID) using a modified Thermo Scientific Orbitrap Elite mass spectrometer.