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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-50（4C-O1-1225）
Large-Scale Libraries of Highly Specific Kinase Substrate Peptides for LC/MS-Based Kinome Profiling
(1Kyoto Univ, 2NCVC, 3NIBIOHN)
oJunqi Liang1, Saki Toi1, Junna Nakazono1, Dai Sakamoto1, Naoyuki Sugiyama1,2, Yasushi Ishihama1,3
Interrogation of large-scale kinase activities (kinome profiling) has become crucial because changes in their activity are usually associated with the onset and progression of various diseases. However, the complexity of kinase-substrate relationships under physiological conditions presents a significant challenge for kinome profiling from phosphoproteome data using LC/MS. This process can be significantly simplified by monitoring the phosphorylation status of artificial substrates spiked into the cell lysates, as changes in their phosphorylation levels reflect the activities of their target kinases.
In this study, a Position-Weight-Matrices (PWMs) based substrate peptide design method was utilized for the development of highly sensitive and highly selective kinase substrate peptides. The sensitivity and the selectivity of the designed substrate peptides were evaluated through in vitro kinase assay combined with LC/MS-based quantification.