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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-43
Development of a Quantitative Analysis Method for Blood Metabolites Using a Multi-HPLC Analysis System for Accurate Health Monitoring
(1MIB,Kyushu Univ., 2Shimadzu)
oKotaro Harada1, Masatomo Takahashi1, Shoji Shinadama2, Keisuke Nakata1, Kazuki Ikeda1, Maiko Goto1, Yoshihiro Hayakawa2, Takeshi Bamba1, Yoshihiro Izumi1
Glucose, neutral lipids, and cholesterol in human blood are commonly used as biomarkers in health screening blood tests. Because the blood concentrations of these metabolites are relatively high, a simple and highly reproducible measurement method has been established. On the other hand, to realize personalized medicine in the future, the expansion of highly reliable blood biomarkers and the development of measurement methods are issues that need to be addressed. Here, we have developed a multi-high-performance liquid chromatography (HPLC) analysis system that can continuously analyze three classes of compounds, including sugars, organic acids, and amino acids, with high repeatability and quantitative accuracy. However, the ability to identify compounds by HPLC analysis alone is limited. Therefore, in this study, we developed a new analysis system that combines HPLC with a preparative device and high-resolution tandem mass spectrometry technology to estimate the structures of unidentified compounds obtained by HPLC analysis. The multi-HPLC analysis method has excellent repeatability and quantitative performance, and by proceeding with the structural determination of unidentified peaks, it will be possible to make more comprehensive quantitative measurements of blood metabolites. This analytical method is expected to be applied to the discovery of new disease biomarkers and future precision health monitoring.