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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-30
Development of a Nano-ESI-MS/MS Method with the In-Capillary Derivatization for the Single-Cell Amino Acid Metabolomics
(1Univ. Shizuoka, 2Meijo Univ., 3Ajinomoto)
oAogu Furusho1, Daiki Hosojima1, Yukino Yamaguchi1, Hajime Mizuno1,2, Sachise Karakawa3, Kotoe Nakasha3, Akihiro Arakawa3, Akiho Murai3, Eiji Sugiyama1,2, Kenji Kojima1, Kenichiro Todoroki1
A nano-ESI-MS/MS method combined with the in-capillary derivatization was developed for the single-cell amino acid metabolomics. Due to the cell heterogeneity in the tumor tissues and the cultured cells, the metabolomics study at the single-cell level is required to further elucidate the biological phenomena. In this research, a single cell was captured into a glass capillary followed the in-capillary derivatization for the highly sensitive and selective analysis of intracellular amino acids. The derivatized amino acids in the capillary were then directly detected by nanoESI-MS/MS. The derivatization reagents and their reaction conditions were investigated, and amino acids with the optimal reagent were detected in the three cultured cell lines. Further, the non-targeted amino acid analysis showed the possibility to distinguish the different cell lines by a single-cell measurement. The present method is a promising tool to accomplish the single-cell metabolomics.