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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-29
Direct Detection of Vitamin D Analogue by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry
(1Toyama. Pref. Univ., 2Kanazawa Univ., 3Hamamatsu Photonics)
oChouma Kurihashi1, Shigehiro Karashima2, Takamasa Ikeda3, Issey Osaka1
Vitamin D is obtained from dietary sources or synthesized in the human skin after exposure to sunlight. It undergoes sequential hydroxylation in the liver and kidneys, resulting in 25-hydroxyvitamin D (25(OH)D) and active vitamin D, 1ɑ,25-dihydroxyvitamin D (1,25(OH)2D). Due to low ionization efficiency, derivatization of vitamin D is required in LC/MS and MALDI/MS measurements. In this study, surface-assisted laser desorption/ionization (SALDI)/mass spectrometry (MS) with platinum (Pt) materials was evaluated for the direct detection of vitamin D. We utilized Pt film formed by sputtering and a Pt-coated porous plate formed of glass beads,1) Poropare™, as substrate materials for SALDI. Since Poropare transfers the target components to its surface, tissue sectioning is not required, allowing for easier MS imaging. In the SALDI/MS experiments, the Na+-adducted ions of vitamin D3 and its analogues were detected directly. The direct detection of vitamin D3 and its analogues have indicated the possibility of MS imaging of vitamin D3 without derivatization. This approach facilitates the elucidation of disease mechanisms and the metabolic analysis of functional foods.