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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-24
Development of a Simplified Estrogen Analysis Method: Liquid-Liquid Extraction and Derivatization Strategy Comparison
(1Kangwon Nat'l Univ., 2Konkuk Univ.)
oMin-Ho Song1, Ji-Woo Yu1,2, Jung-Hoon Lee1, Eun-Song Choi1, Ji-Ho Lee1
Quantitative analysis of estrogens in biological samples is essential for clinical and environmental research, as estrogens play a crucial role in endocrine regulation and hormone-related disorders. Accurate measurement is critical for diagnosing hormonal imbalances and assessing the impact of endocrine-disrupting chemicals in the environment. Traditionally, solid-phase extraction has been used to separate non-conjugated estrogens from their glucuronide- and sulfate-conjugated forms. However, this method required multiple processing steps and specialized cartridges, making it labor-intensive. Additionally, derivatization with dansyl chloride was crucial for enhancing detection sensitivity in estrogen analysis. Two major derivatization approaches exist: one using acetone and aqueous potassium carbonate, which selectively derivatizes phenolic hydroxyl (-OH) groups, and another employing dichloromethane, N,N-diisopropylethylamine, and 4-dimethylaminopyridine, which derivatizes all hydroxyl (-OH) groups. Despite their differences, no systematic study has compared the efficiency of these two derivatization methods. This study introduced a simplified liquid-liquid extraction method for the efficient separation and analysis of free and conjugated estrogens. Also, derivatization techniques were evaluated and compared by optimizing reaction method, time and reagent concentration. The findings aim to establish a more efficient and reproducible method for estrogen quantification, thereby improving analytical precision and reliability.