| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-21
Compositional Analysis of Protein Corona on Diamond Nanoparticles Using Mass Spectrometry
(1Physics / NDHU, 2Biochem / NDHU, 3IAMS, 4TCU)
oMhikee Descanzo1, Yu-Chung Chen1, Ming-Chi Chung2, Nguyen Nghiem Bich Ngoc2, Po-Chi Soo4, Ruey-Yi Chang2, Chia-Liang Cheng1, Huan-Chen Chang3, Wen-Ping Peng1
Nanodiamonds (NDs) are promising agents for various biomedical applications. Upon entering the body, NDs interact with proteins, forming a "protein corona" (PC) that influences their biological fate and cytotoxicity. This study investigates how the PC evolves on two types of NDs: oxidized detonation ND (oxDND) and high-pressure high-temperature ND (HPHT ND). The results show that PC formation depends on serum protein abundance, protein affinity, kinetics, and ND properties. At higher serum concentrations, both NDs are predominantly coated with apolipoprotein A1 (APO A1). As serum concentration decreases, oxDND is dominated by human serum albumin (HSA), while HPHT ND forms a mixed APO A1-HSA corona. The larger pore diameter of oxDND (9.41 nm) allows access to larger proteins, such as HSA, whereas HPHT ND's smaller pore (3.39 nm) favors low molecular weight proteins. Notably, oxDND with an HSA-dominant corona shows reduced toxicity toward A549 cells, while HPHT with a mixed APO A1-HSA corona exhibits higher cytotoxicity. These findings emphasize the complex role of the corona in ND toxicity and highlight the need for further research to understand how specific proteins may alleviate or intensify cytotoxicity.