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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-PM-18
High Resolution DESI imaging Single Cell Analysis
(1Nihon Waters, 2Waters, 3University of Surrey)
oMotoji Oshikata1, Thanai Paxton1, Mark Towers2, Gary Harland2, Scarlet Ferrinho2, Lisa Towers2, Lee Gethings2, Preeti Mourya3, Shazneil Briones3, Olivier Cexus3, Paul Townsend3, Joanne Ballantyne2
Desorption electrospray ionization (DESI) is an established imaging technique allowing imaging of surfaces with minimal sample preparation.
As with all imaging techniques there is a continual drive towards smaller pixel sizes resulting in a higher image resolution. Here we demonstrate how using a low flow DESI technique, on a commercially available DESI source setup, allows the visualization of single cells within tissue and within non-confluent cultures.
It has been recently discovered that morphologically and genetically identical cells are found to exhibit heterogeneity on transcriptomic, proteomic, metabolomic and lipidomic levels. To investigate population heterogeneity, single cell analysis is becoming increasingly vital. Achieving single cell resolution within DESI imaging applications allows these biological variations to be visualized on a cell-by-cell basis.