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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-48
Automatic Sample Additive Scans for Electrospray Ionization Mass Spectrometry
(NTHU CHEM)
oI-Ting Wu, Decibel Elpa, Pawel Urban
In order to achieve optimum conditions for electrospray ionization mass spectrometry (ESI-MS) methods, samples are often supplemented with acids, bases, supercharging agents, and electrospray-friendly solvents. Typically, one specific pH or concentration of these additives is used in a given method, which is selected based on optimization or literature. However, different pH and concentrations of additives can be suitable for analysis of different species, and can bring different analytical information. Therefore, here we demonstrate a setup for dynamic scans of acid-base composition, solvent, and supercharging agent concentrations in ESI-MS. For low-molecular-weight analytes, MS signals can be enhanced by selecting optimum conditions in a single scan. For proteins, charge state distributions can be manipulated, revealing information about the vulnerability of protein tertiary structures to changing chemical environments. The setup has been constructed using off-the-shelf components and controlled via a simple Python script. It offers a cost-effective and flexible approach for enhancing ESI-MS analysis. By integrating dynamic scanning into MS workflows, this setup facilitates rapid optimization of experimental conditions, improves analytical sensitivity, and enhances structural characterization of biomolecules, making it a valuable tool for advancing ESI-MS applications.