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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-39
Fundamental study of LC/MS/MS analytical conditions for tryptophan-derived metabolites in Niemann-Pick Disease Type C model cells
(1Tohoku Univ., 2Tohoku Univ. Hosp.)
oMasahiro Watanabe1, Masamitsu Maekawa1,2, Nariyasu Mano1,2
Niemann-Pick disease type C (NPC) is a rare disorder characterized by defects in cholesterol transport, leading to neurological symptoms. A wide range of tryptophan-derived metabolites has been implicated in NPC pathology. Therefore, elucidating fluctuations in these metabolites is crucial for a better understanding of NPC pathophysiology. This study aimed to optimize chromatographic conditions for their retention, detection, and separation.
We evaluated CAPCELL PAK ADME-HR (ADME), INERT ADME-HR (i-ADME), and InertSustain AQ-C18 (AQ-C18) columns for the analysis of 17 tryptophan-derived metabolites using LC-MS/MS with a Triple Quad 7500 mass spectrometer. Calibration curves were constructed for quantification.
ADME and i-ADME columns exhibited poor peak shape and unstable retention times for quinolinic acid and picolinic acid. In contrast, an AQ-C18 column improved peak resolution, increasing peak height and the signal-to-noise ratio by more than tenfold. Calibration curves were successfully generated for 15 metabolites (R2 > 0.99), but not for quinolinic and picolinic acids because these analytes were not retained in some measurements.
While an AQ-C18 column improved chromatographic performance, retention issues remained for quinolinic and picolinic acids. Future work will focus on stabilizing retention times and refining calibration curves for a comprehensive metabolic analysis of NPC models.