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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-38
Unveiling the Overall Triglyceride Composition in Skin through Optimized Monophasic Sebutape Extraction and Pseudo-Targeted Lipidomics Strategy
(Univ. Taiwan)
oYa-Chu Kuo, Ching-Hua Lee, Ching-Hua Kuo
We developed an efficient lipid extraction method from Sebutape to explore the lipid profile of the skin. Traditional liquid-liquid extraction (LLE) methods, such as Folch and MTBE, had certain limitations when extracting from Sebutape. For example, chloroform could dissolve the Sebutape polymer, and the biphasic extraction process often led to the loss of some lipid components. To address this, we optimized a monophasic solvent approach using ethanol (EtOH), which successfully extracted triglycerides (TG), diglycerides (DG), and ceramides (Cer) while minimizing polymer degradation, making it the ideal solvent for Sebutape lipid extraction.
Furthermore, our analysis revealed significant differences in TG composition between skin and plasma, with skin TGs containing a higher proportion of short-chain and odd-chain fatty acids, suggesting a unique lipid profile in the skin. To further investigate these differences, we employed a pseudo-targeted lipidomics strategy utilizing multiple reaction monitoring (MRM) to target specific fatty acid chains. This approach offered a more detailed and comprehensive analysis of TG species compared to data-dependent acquisition (DDA) methods.
In summary, our optimized ethanol-based extraction method, combined with a pseudo-targeted lipidomics strategy, provides an efficient and reliable approach for Sebutape lipid analysis, enabling a deeper understanding of skin lipidome differences.