| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-37
Search for Differentiation markers of human iPSCs by EV using data-independent acquisition LC/MS/MS
(Yokohama Univ.)
oMei Mikami, Shunsuke Hoshina, Eri Katsuno, Kansei Takashita, Daisuke Takakura, Nana Kawasaki
Human induced pluripotent stem cells (iPSCs) are expected to have clinical applications for regenerative medicine. Reliable and effective quality control methods for differentiating iPSCs are required. A quality control method using extracellular markers secreted by cells into culture supernatants represents a new strategy. We focused on extracellular vesicles (EVs) that could be concentrated. In this study, we analyzed the changes in EV proteins during iPSC differentiation by data-independent acquisition LC/MS/MS (DIA-MS), which is highly quantitative and comprehensive, to identify new extracellular secreted differentiation markers. Human iPSCs were cultured and differentiated into neural stem cells (NSCs). The cell culture medium and cell samples for each undifferentiated condition and after differentiation was collected. The EVs were concentrated by affinity purification. The digested EV and cell samples were analyzed by DIA-MS. Proteomics analysis was performed using DIA-NN software. 1732 proteins overlapping between the EV samples and the cell samples. We focused on FXYD6 and TMEFF1, which were proteins exhibiting significantly a large fold-change in the EV and cell samples. This analysis is the first to identify them as candidate marker proteins. We plan to construct a quantitative evaluation system that focuses on these proteins for the differentiation of iPSCs using flow cytometry.