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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-35
Search for Growth Factors Secreted from Mesenchymal Stem Cells
(Yokohama City Univ.)
oYuzuka Kuba, Tohru Sugawara, Nana Kawasaki
Mesenchymal stem cells (MSCs) from bone marrow (BM) and adipose tissue (AD) exhibit neuroprotective and immunomodulatory effects through their secretome. Their proliferative capacity varies by tissue origin, donor source, and cell passage, influencing therapeutic efficacy. This study used DIA-MS to identify secreted proteins associated with MSCs proliferation.
Three BM-MSC lines and three AD-MSC lines were cultured for 24 h under simulated hypoxia and ischemic conditions at early passage (P4) and late passage (P8).
The proliferative capacity of P4 was higher than that of P8 in all cell lines, particularly in cell line A. In total, 1,224 proteins were identified in the culture supernatants, and 2,677 proteins were identified within the cells. Specifically, 300 proteins were identified in the secretome. Seven proteins were upregulated in P4 of BM compared to P8 and twelve proteins in P4 of AD. Similarly, thirty-five proteins were upregulated from P4 of cell line A compared with other BM-derived samples. Among them, Multiple EGF-like Domain 8 (MEGF8) was commonly upregulated.
This suggests that tissue origin may influence the therapeutic efficacy of MSCs and that certain proteins, including MEGF8, may regulate cell proliferation. Future research will introduce the identified proteins into MSCs to verify their role in proliferation.