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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-26
Enhanced Proteomic Profiling through Dual-Labeling BioID-MS Targeting the ER and Golgi Apparatus
(1CityU, 2HKSMS)
oFenglian Yang1,2, Liang Zhang1,2
We have developed an innovative dual-labeling application of the proximity-dependent biotin labeling technology, BioID-MS, targeting both the endoplasmic reticulum (ER) and Golgi apparatus. This approach enables proteins traversing the ER or Golgi to be tagged with biotin, facilitating subsequent enrichment using beads and analysis via mass spectrometry. We compared three BioID labeling strategies: ER-only, Golgi-only, and combined ER-Golgi labeling, analyzing both cellular and supernatant levels. Our results demonstrate that the combined ER-Golgi labeling yields a higher total protein count compared to the sum of individual ER and Golgi labeling, highlighting the method's superiority in providing a more comprehensive proteomic profile and increased sensitivity in detecting transient and low-abundance proteins. Furthermore, applying this technique to a mouse obesity model revealed distinct proteomic alterations between obese and lean phenotypes, underscoring its potential for elucidating protein dynamics in various biological contexts. This dual-labeling BioID-MS method not only enhances the identification of proteins but also offers a robust tool for studying the spatial and temporal dynamics of protein interactions and modifications within the secretory pathway. The ability to capture a broader spectrum of proteins passing through the ER and Golgi provides deeper insights into cellular processes and disease mechanisms, making it a valuable approach for future proteomic studies.