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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-22
Development of a Pin-point Probe ESI-MS system with Robotic Automation
(1HDU/Univ. of Yamanashi, 2HDU, 3Univ. of Yamanashi)
oLei Li1, Qiangqiang Xie2, Lee Chuin Chen3, Satoshi Ninomiya3
An automatic sampling system for probe-ESI mass spectrometry using a three-axis robot using a touch sensor has previously been developed for sampling liquid loaded in a 96-well or 384-well plate.1),2) In that system, a metallic plate applied with a high-frequency activation voltage needed to be placed beneath the insulating sample plate to sense the contact between the sampling probe and the liquid. The coordinates of the sample plate and the ESI emitter also need to be reset after the changing to new plate position and the interchanging ESI probe. To handle samples of arbitrary shape and size and automatic coordinate setting, a new system is developed in this work using a different touch-sensing design and a camera system for the global positioning of the sample and probe emitter. The touch-sensing mechanism involves a disc electrode attached to the emitter probe that is fed with r.f. signal (500 kHz and 5 Vpp). A contact between the probe and the liquid or wet sample causes an impedance change between the disc electrode and the ESI probe that can be detected by a amplifying and heterodyne detection circuit. The position of the ESI probe is controlled by a PC that sends commands to the three-axis robot. With the combination of image processing using OpenCV, the ESI probe is designed to move to the pin-pointed position designated by the user on the monitor screen.