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Plenary Lectures Day1　Day2　Day3　Day4

MSSJ Award Lectures Day1

Oral Sessions Day2　Day3　Day4

Poster Presentations Day1　Day2　Day3　Day4

Late-Breaking Posters Day1

Luncheon Seminars Day2　Day3　Day4

Teatime Sessions Day3

Corporate Posters Day2　Day3　Day4

Corporate Program

Poster Presentations

Day 3, June 24（Tue.）　

Room P (Maesato East, Foyer, Ocean Wing)

• 3P-AM-19
• PDF

Development of a Compact t-SPESI System for High-sensitivity Mass Spectrometry Imaging of Biological Tissue

(¹Grad. Sch. Sci., Univ. Osaka, ²FRC, Univ. Osaka, ³Grad. Sch. Eng., Univ. Osaka)
^oYoichi Otsuka^1,2, Takao Yasuda¹, Mengze Sun¹, Zhou Yang¹, Shuichi Shimma³, Michisato Toyoda^1,2

Visualization of cellular heterogeneity in biological tissues is crucial for understanding disease mechanisms and identifying biomarkers. Mass spectrometry imaging (MSI) enables spatial analysis of biomolecules, and recent advancements have focused on achieving single-cell resolution. However, a major challenge in single-cell MSI (SC-MSI) is to improve ion detection sensitivity while maintaining spatial resolution.
We developed a compact tapping-mode scanning probe electrospray ionization (t-SPESI) system designed to enhance ion detection sensitivity. This system integrates an inverted microscope and automated stages within a compact frame to optimize ion transfer efficiency. This design minimizes ion loss and improves the overall detection sensitivity, making it more suitable for high-resolution MSI applications.
In this presentation, we will discuss the advantages of our compact t-SPESI system, its application in biological tissue analysis, and its implications for SC-MSI. This development represents a significant step toward more sensitive and precise MSI for studying cellular heterogeneity and disease-related biomolecular changes.

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