Abstract

Poster Presentations

Day 3, June 24（Tue.）　

Room P (Maesato East, Foyer, Ocean Wing)

3P-AM-18
PDF

Determination of Benzophenone-type UV filters in Urine of Taiwanese Young Adults by Liquid-Liquid Extraction-based UHPLC–MS/MS

(¹NYCU, ²NYC Univ.)
^oSi-Yu Liu¹, Yu-Fang Huang²

Benzophenones (BPs) are widely used as photoinitiators and ultraviolet (UV) stabilizers in a variety
of everyday products. They are commonly used in printing inks for food packaging materials, personal
care products, cosmetics, and sunscreens to protect the skin from UV damage such as sunburn and skin
cancer. BP, an emerging contaminant, is one of the endocrine-disrupting chemicals and is classified as a
probable human carcinogen (2B). Exposure to BPs can occur through food ingestion, skin contact, or
inhalation. This study describes a method for determining five BPs (BP-1, BP-2, BP-3, BP-8, and 4-
OHBP) in human urine by liquid-liquid extraction coupled with ultra-high performance liquid
chromatography-tandem mass spectrometry (UHPLC-MS/MS) 1) .
The method featured a satisfactory linearity (R 2 > 0.995), low detection limit (0.03-0.11 ng/mL), and
favorable precision. The mean recovery (n=5) range was 97–106% with an intraday precision range of
1.6–7.3% at the level of 50 ng/mL. The validated method was employed for the analysis of Taiwanese
young adults (N=200). Among the samples analyzed, BP-3 and BP-1 were detected in 22% and 5%
respectively. The mean (range) of BP-3 was higher at 14.3 (0.1–659.9) ng/mL, followed by BP-1 at 8.4
(0.1–85.9) ng/mL. The simple and fast method is suitable for routine analysis of BPs compounds in
human urine and may be useful for urine biomonitoring.