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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-14
Quantitative Peptidomics Revealed a Novel Peptide Cytokine for Eliciting Plant Immunity by Pathogen-Associated Molecular Pattern
(1ABRC, Academia Sinica, 2IOB, National Taiwan Univ.)
oSheng-Chi Hung1,2, Kai-Ting Fan1, Jia-Wei Hsu1, Yet-Ran Chen1,2
To survive from unfavourable environments such in biotic or abiotic stresses, plants have developed complex signalling mechanisms to orchestrate the physiology of plant cells for diverse stress responses, with peptide cytokines playing a central role in these processes. However, the dynamic expression and low abundance (sub-nM levels) of these biomolecules present challenges for studying their regulation. To address this, we developed a high-throughput approach for identifying and quantifying peptide cytokines under specific stress conditions. Our research uncovered a novel peptide, we named it "FIKA1", which is induced by the Pathogen Associated Molecular Pattern (PAMP) flagellin peptide flg22. FIKA1 elicits defense responses and induce both local and systemic immunity against Pseudomonas syringae pv. tomato DC3000. Quantitative proteomics demonstracted that FIKA1 activates defense-related proteins and induces the accumulation of PR1, which encodes a cytokine crucial for systemic immunity at its C-terminal end, called CAPE9. Our findings suggest that FIKA1-mediated systemic immunity is depended on PR1/CAPE9, with FIKA1 involving in the activation of CAPE9 maturation mechanism from PR1. This study highlights the potential of our peptidomic approach for identifying stress-induced peptides and advances our understanding of the plant immunity.