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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-13
Quantitative proteomics using high-sensitivity data-independent acquisition
(1K.K. AB SCIEX, 2SCIEX, Canada)
oTakeshi Shibata1, Ushio Takeda1, Ihor Batruch2, Patrick Pribil2
Proteomics identifies and quantifies proteins to reveal disease-related abundance changes. Data-independent acquisition (DIA) is a standard technique due to its robustness and reproducibility. A key challenge is detecting low-abundance proteins, requiring improved mass spectrometry (MS) sensitivity, particularly MS/MS. This study evaluates a high-sensitivity DIA-based workflow using Zeno SWATH DIA on a novel QTOF platform. Compared to its predecessor, the new system exhibited ~5× higher MS/MS signal intensity, enabling the identification of more peptides from smaller sample injections. Increased sensitivity led to over 150% more quantifiable peptides and proteins, especially at low on-column loadings. The novel QTOF platform also includes software enhancements for automated method optimization, guided MS tuning, and real-time performance monitoring, improving proteomics research efficiency and accuracy.