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Plenary Lectures Day1　Day2　Day3　Day4

MSSJ Award Lectures Day1

Oral Sessions Day2　Day3　Day4

Poster Presentations Day1　Day2　Day3　Day4

Late-Breaking Posters Day1

Luncheon Seminars Day2　Day3　Day4

Teatime Sessions Day3

Corporate Posters Day2　Day3　Day4

Corporate Program

Poster Presentations

Day 3, June 24（Tue.）　

Room P (Maesato East, Foyer, Ocean Wing)

• 3P-AM-12
• PDF

An Easy Workflow for the Characterization and Relative Quantification of Recombinant Adeno-Associated Viruses (rAAVs) using Charge Detection Mass Spectrometry

(¹Nihon Waters, ²Waters)
^oKenji Hirose¹, Etsuko Yada¹, Anisha Haris², Rebecca D'esposito², Kevin Giles²

As AAV-based gene therapy evolves, improved analytical tools are needed to better characterize therapeutics and facilitate production. All AAV capsid particle populations, including impurities, require characterization due to their impact on clinical efficacy, safety, and immunogenicity. Charge detection mass spectrometry (CDMS), using an electrostatic linear ion trap (ELIT), is a cutting-edge technique for ultra-high mass analysis. It measures the mass-to charge ratio (m/z) and charge (z) of individual ions, enabling mass calculation. We demonstrate how dedicated CDMS software streamlines workflows for the assignment and relative quantification of AAV capsids, ensuring accuracy and efficiency in data analysis, ultimately supporting and improving analytical productivity required for AAV characterization during the manufacturing process.

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